PUBLICATION

Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity

Authors
Ballim, R.D., Nagao, Y., Kelsh, R.N.
ID
ZDB-PUB-190413-7
Date
2019
Source
Methods in molecular biology (Clifton, N.J.)   1976: 195-206 (Chapter)
Registered Authors
Kelsh, Robert, Nagao, Yusuke
Keywords
Iridophore, Leucophore, Live imaging, Medaka, Melanocyte, Neural crest, Pigment cells, Tol2, Transient transgenesis, Xanthophore, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Neural Crest/cytology*
  • Oryzias
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed
30977075 Full text @ Meth. Mol. Biol.
Abstract
Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes