Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity
- Ballim, R.D., Nagao, Y., Kelsh, R.N.
- Methods in molecular biology (Clifton, N.J.) 1976: 195-206 (Chapter)
- Registered Authors
- Kelsh, Robert, Nagao, Yusuke
- Iridophore, Leucophore, Live imaging, Medaka, Melanocyte, Neural crest, Pigment cells, Tol2, Transient transgenesis, Xanthophore, Zebrafish
- MeSH Terms
- Animals, Genetically Modified
- Cell Differentiation/genetics
- Cell Differentiation/physiology
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology
- Neural Crest/cytology*
- Zebrafish Proteins/metabolism
- 30977075 Full text @ Meth. Mol. Biol.
Ballim, R.D., Nagao, Y., Kelsh, R.N. (2019) Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity. Methods in molecular biology (Clifton, N.J.). 1976:195-206.
Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes