PUBLICATION
Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva
- Authors
- Kumari, S., Mazumder, A.G., Bhardwaj, A., Singh, D.
- ID
- ZDB-PUB-190413-2
- Date
- 2019
- Source
- Prostaglandins, leukotrienes, and essential fatty acids 143: 15-20 (Journal)
- Registered Authors
- Keywords
- Clonus-like seizures, Omega-3 fatty acids, Pentylenetetrazol, Polyunsaturated fatty acids, Zebrafish, c-fos
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Convulsants/adverse effects
- Convulsants/pharmacology*
- Dietary Supplements
- Disease Models, Animal
- Docosahexaenoic Acids/metabolism
- Epilepsy/diet therapy
- Female
- Gene Expression/drug effects
- Larva/drug effects*
- Male
- Pentylenetetrazole/adverse effects
- Pentylenetetrazole/pharmacology*
- Proto-Oncogene Proteins c-fos/genetics
- RNA, Messenger/genetics
- Seizures/chemically induced*
- Swimming
- Zebrafish/embryology*
- alpha-Linolenic Acid/metabolism
- alpha-Linolenic Acid/pharmacology*
- PubMed
- 30975378 Full text @ Prostaglandins Leukot. Essent. Fatty Acids
Citation
Kumari, S., Mazumder, A.G., Bhardwaj, A., Singh, D. (2019) Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva. Prostaglandins, leukotrienes, and essential fatty acids. 143:15-20.
Abstract
Over the past few years, there has been a tremendous increase in interest of general population toward food-based therapies for management of chronic clinical conditions due to their lesser adverse effects with prolonged use over pharmacotherapies. Foods enriched with omega-3 fatty acids have shown some promising results in case of epilepsy. The present study was envisioned to investigate the effect of early exposure of α-linolenic acid (ALA), an essential omega-3 fatty acid in developing zebrafish (Danio rerio) embryos toward pentylenetetrazol (PTZ)-induced seizure susceptibility. The healthy wild-type zebrafish embryos were incubated in system water or system water containing different ALA concentrations (1-20 µM) till 7 dpf (days post fertilization). Each larva at 7 dpf was placed in 8 mM PTZ solution and seizure event was recorded. ALA incubation at 10 µM and 20 µM concentrations showed a dose-dependent reduction in PTZ-mediated hyperactive responses in larvae indicated by a marked decrease in total distance travelled and speed, as compared to vehicle control. Furthermore, both the treated groups showed increase in the latency to PTZ-induced clonus-like seizures in larvae, as compared to vehicle control. ALA incubated larvae at 10 µM and 20 µM concentrations also showed a significant reduction in c-fos mRNA level. A marked increase in the level of ALA and docosahexaenoic acid was also observed in the larvae incubated at highest effective concentration of ALA. The present study concluded that embryonic exposure of ALA reduced PTZ-induced seizures in zebrafish larva.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping