PUBLICATION

Knockout of von Willebrand factor in Zebrafish by CRISPR/Cas9 mutagenesis

Authors
Iyer, N., Tcheuyap, V.T., Schneider, S., Marshall, V., Jagadeeswaran, P.
ID
ZDB-PUB-190411-6
Date
2019
Source
British journal of haematology   186(4): e76-e80 (Journal)
Registered Authors
Jagadeeswaran, Pudur
Keywords
CRISPR/Cas9, Zebrafish, bleeding, knockout, laser-induced thrombosis, von Willebrand factor
MeSH Terms
  • von Willebrand Factor/genetics*
  • Exons
  • Gene Knockout Techniques
  • CRISPR-Cas Systems*
  • Mutagenesis*
  • RNA, Guide, Kinetoplastida
  • Gene Targeting
  • Zebrafish/genetics*
  • Sequence Analysis, DNA
  • Gene Editing*
  • Animals
(all 11)
PubMed
30968393 Full text @ Br. J. Haematol.
Abstract
Genes / Markers
Figures
No images available
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
vwfunt2/+light ablation: bloodDay 5
vwfunt2/+light ablation: bloodDay 5
vwfunt2/+physical alteration: caudal veinDay 5
vwfunt2/unt2light ablation: bloodDay 5
vwfunt2/unt2light ablation: bloodDay 5
vwfunt2/unt2light ablation: bloodDay 5
vwfunt2/unt2physical alteration: caudal veinDay 5
1 - 7 of 7
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
unt2
    		Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    Human Disease Fish Conditions Evidence
    von Willebrand's diseasevwfunt2/+light ablation: bloodTAS
    von Willebrand's diseasevwfunt2/+physical alteration: caudal veinTAS
    1 - 2 of 2
    Show
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    vwfCRISPR1-vwfCRISPR
    1 - 1 of 1
    Show
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Iyer, N., Tcheuyap, V.T., Schneider, S., Marshall, V., Jagadeeswaran, P. (2019) Knockout of von Willebrand factor in Zebrafish by CRISPR/Cas9 mutagenesis. British journal of haematology. 186(4):e76-e80.