PUBLICATION
Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish
- Authors
- Jarque, S., Ibarra, J., Rubio-Brotons, M., García-Fernández, J., Terriente, J.
- ID
- ZDB-PUB-190411-2
- Date
- 2019
- Source
- International Journal of Molecular Sciences 20(7): (Journal)
- Registered Authors
- de Txintxetru, Jone Ibarra Otsoa, Fernandez, Jessica Garcia, Ortiz, Sergio Jarque, Terriente, Javier
- Keywords
- androgens, endocrine disruption, estrogens, thyroid, transcriptomics, zebrafish
- MeSH Terms
-
- Androgens/analysis
- Animals
- Biological Assay/methods*
- Endocrine Disruptors/toxicity*
- Endocrine System/drug effects
- Endocrine System/metabolism
- Estrogens/analysis
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Transcriptome/genetics*
- Zebrafish
- PubMed
- 30965663 Full text @ Int. J. Mol. Sci.
Citation
Jarque, S., Ibarra, J., Rubio-Brotons, M., García-Fernández, J., Terriente, J. (2019) Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish. International Journal of Molecular Sciences. 20(7):.
Abstract
Small fish are an excellent experimental model to screen endocrine-disrupting compounds, but current fish-based assays to detect endocrine disruption have not been standardized yet, meaning that there is not consensus on endpoints and biomarkers to be measured. Moreover, exposure conditions may vary depending on the species used as the experimental model and the endocrine pathway evaluated. At present, a battery of a wide range of assays is usually needed for the complete assessment of endocrine activities. With the aim of providing a simple, robust, and fast assay to assess endocrine-disrupting potencies for the three major endocrine axes, i.e., estrogens, androgens, and thyroid, we propose the use of a panel of eight gene expression biomarkers in zebrafish larvae. This includes brain aromatase (cyp19a1b) and vitellogenin 1 (vtg1) for estrogens, cytosolic sulfotransferase 2 family 2 (sult2st3) and cytochrome P450 2k22 (cyp2k22) for androgens, and thyroid peroxidase (tpo), transthyretin (ttr), thyroid receptor α (trα), and iodothyronine deiodinase 2 (dio2) for thyroid metabolism. All of them were selected according to their responses after exposure to the natural ligands 17β-estradiol, testosterone, and 3,3',5-triiodo-L-thyronine (T3), respectively, and subsequently validated using compounds reported as endocrine disruptors in previous studies. Cross-talk effects were also evaluated for all compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping