PUBLICATION
Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii
- Authors
- Kang, M.C., Lee, H., Choi, H.D., Jeon, Y.J.
- ID
- ZDB-PUB-190331-7
- Date
- 2019
- Source
- International journal of biological macromolecules 132: 142-149 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Anti-oxidant, Oxidative stress, Polysaccharides, Sargassum thunbergii, Zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/chemistry*
- Antioxidants/isolation & purification
- Antioxidants/pharmacology*
- Cell Survival/drug effects
- Chlorocebus aethiops
- Enzymes/metabolism*
- Hydrogen Peroxide/metabolism
- Intracellular Space/drug effects
- Intracellular Space/metabolism
- Monosaccharides/analysis
- Polysaccharides/chemistry*
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology*
- Sargassum/chemistry*
- Sargassum/metabolism
- Sulfates/chemistry*
- Vero Cells
- Zebrafish
- PubMed
- 30926508 Full text @ Int. J. Biol. Macromol.
Citation
Kang, M.C., Lee, H., Choi, H.D., Jeon, Y.J. (2019) Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii. International journal of biological macromolecules. 132:142-149.
Abstract
The protective effect of sulfated polysaccharides (PS-4) isolated from Sargassum thunbergii against oxidative stress in cells and zebrafish was investigated. To begin, a variety of enzymatic extraction methods were used, including five carbohydrases and four proteases. Among the enzymatic extracts, the S. thunbergii Celluclast-assisted extract (STC) demonstrated the strongest scavenging activities against hydrogen peroxide (H2O2). Five polysaccharide fraction (PS-1 to 5) were isolated from STC and purified using ion exchange column chromatography. The antioxidant activity of these fractions was then assessed. PS-4 demonstrated the most promising free radical scavenging and antioxidant activity. PS-4 had a higher content of sulfated polysaccharide than STC. Thus, increasing the proportion of sulfated polysaccharides may be attributed to protection against H2O2-mediated cell damage and oxidative stress in cell lines and in zebrafish. These results suggest that PS-4 could be a potent antioxidant agent in functional foods and cosmetics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping