PUBLICATION

Genetic control of cellular morphogenesis in Müller glia

Authors
Charlton-Perkins, M., Almeida, A.D., MacDonald, R.B., Harris, W.A.
ID
ZDB-PUB-190330-8
Date
2019
Source
Glia   67(7): 1401-1411 (Journal)
Registered Authors
Harris, William A.
Keywords
CRISPR, Müller glia, morphogenesis, transcriptome, zebrafish
Datasets
GEO:GSE120275
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/physiology
  • Clustered Regularly Interspaced Short Palindromic Repeats/physiology
  • Ependymoglial Cells/physiology*
  • Gene Expression Profiling/methods*
  • Morphogenesis/physiology*
  • Neurogenesis/physiology*
  • Neuroglia/physiology*
  • Transcriptome/physiology*
  • Zebrafish
PubMed
30924555 Full text @ Glia
Abstract
Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell-specific morphology remain largely unexplored. To identify the genes involved in the morphogenesis of the principal glial cell type in the vertebrate retina, the Müller glia (MG), we used genomic and CRISPR based strategies in zebrafish (Danio rerio). We identified 41 genes involved in various aspects of MG cell morphogenesis and revealed a striking concordance between the sequential steps of anatomical feature addition and the expression of cohorts of functionally related genes that regulate these steps. We noted that the many of the genes preferentially expressed in zebrafish MG showed conservation in glia across species suggesting evolutionarily conserved glial developmental pathways.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes