ZFIN ID: ZDB-PUB-190322-6
The Metalloproteinase adam19b Is Required for Sensory Axon Guidance in the Hindbrain
Cox, J.A., Voigt, M.M.
Date: 2019
Source: Frontiers in neural circuits   13: 14 (Journal)
Registered Authors: Cox, Jane, Voigt, Mark
Keywords: axon guidance, cranial nerves, metalloprotease, sensory circuits, zebrafish
MeSH Terms:
  • ADAM Proteins/genetics
  • ADAM Proteins/metabolism*
  • Animals
  • Animals, Genetically Modified
  • Axon Guidance/genetics
  • Axon Guidance/physiology*
  • CRISPR-Associated Protein 9/genetics
  • CRISPR-Associated Protein 9/metabolism
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA Mutational Analysis
  • Embryo, Nonmammalian
  • Ganglia, Invertebrate/cytology
  • Gene Expression Regulation, Developmental/physiology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Larva
  • Mutation/genetics
  • Receptors, Purinergic P2/genetics
  • Receptors, Purinergic P2/metabolism
  • Rhombencephalon/cytology*
  • Rhombencephalon/physiology*
  • Sensory Receptor Cells/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 30894803 Full text @ Front. Neural Circuits
Little is known about the molecular and cellular mechanisms involved in the formation of the cranial peripheral sensory system in vertebrates. To identify genes involved in the formation of these circuits, we performed a forward genetic screen utilizing a transgenic zebrafish line (p2rx3.2:gfpsl1) that expresses green fluorescent protein (gfp) in sensory neurons of the Vth, VIIth, IXth and Xth cranial ganglia. Here, we describe a novel zebrafish mutant in which a missense mutation in the adam19b gene selectively affects the epibranchial sensory circuits.