ZFIN ID: ZDB-PUB-190321-9
In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography
Lin, Y., Xiang, X., Chen, T., Gao, C., Fu, H., Wang, L., Deng, L., Zeng, L., Zhang, J.
Date: 2019
Source: Biomedical Optics Express   10: 1184-1195 (Journal)
Registered Authors: Gao, Chuan, Zhang, Jian
Keywords: none
MeSH Terms: none
PubMed: 30891338 Full text @ Biomed. Opt. Express
Because of its similar genetic makeup with humans, zebrafish are an available and well-established osteoporosis model in vivo for anti-osteoporosis drug development as well as the drug safety-evaluation process. However, few optical imaging methods could effectively visualize the bone of adult zebrafish due to their limited penetration depth. In this paper, in vivo high-resolution and long-term characterization of a prednisolone-induced osteoporotic zebrafish model was achieved with spectral-domain optical coherence tomography (SD-OCT). The capability of three-dimensional SD-OCT imaging was also demonstrated in this study. With SD-OCT images, we could non-destructively monitor the deforming process of adult zebrafish skull from several directions at any time. There is good correlation and agreement between SD-OCT and histology. Valuable phenomenon such as bone defects could be quantitatively evaluated using the SD-OCT images at different time points during a period of 21 days.