PUBLICATION
In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish
- Authors
- Chen, M., Huang, R.C., Yang, L.Q., Ren, D.L., Hu, B.
- ID
- ZDB-PUB-190321-15
- Date
- 2019
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33(6): 7721-7733 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- activity, regeneration
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/metabolism*
- Axotomy
- Calcium/metabolism*
- Nerve Regeneration*
- Zebrafish/physiology*
- PubMed
- 30893562 Full text @ FASEB J.
Citation
Chen, M., Huang, R.C., Yang, L.Q., Ren, D.L., Hu, B. (2019) In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(6):7721-7733.
Abstract
Calcium is an important messenger in the neuronal system, but its specific role in axonal regeneration has not been fully investigated. To clarify it, we constructed a noninvasive in vivo calcium-imaging model of zebrafish Mauthner cells and monitored subcellular calcium dynamics during axonal regeneration. Using the calcium indicator GCamp6f, we observed that the regenerative length correlated with the peak amplitude of the evoked calcium response before axotomy, which suggested that the evoked calcium response might serve as a useful indicator of evoked neuronal activity and axonal regenerative capacity. To investigate this possibility, we overexpressed an inward rectifying potassium channel protein (Kir2.1a), to decrease the Mauthner neuronal activity and found that the inhibition of the calcium response correlated with decreased axonal regeneration. In contrast, treatment of pentylenetetrazol and knockout of the sodium voltage-gated channel α subunit 1 gene increased the calcium response and thus enhanced axonal regeneration. Our results therefore increased the understanding of the correlation between the neural activity and the vertebrate axonal regeneration.-Chen, M., Huang, R.-C., Yang, L.-Q., Ren, D.-L., Hu, B. In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping