PUBLICATION
Germline Stem Cells Drive Ovary Regeneration in Zebrafish
- Authors
- Cao, Z., Mao, X., Luo, L.
- ID
- ZDB-PUB-190214-9
- Date
- 2019
- Source
- Cell Reports 26: 1709-1717.e3 (Journal)
- Registered Authors
- Luo, Lingfei
- Keywords
- GSCs, Wnt, nanos2, ovary regeneration, zebrafish
- MeSH Terms
-
- Animals
- Female
- Germ Cells/metabolism*
- Ovary/metabolism*
- Zebrafish
- PubMed
- 30759383 Full text @ Cell Rep.
Citation
Cao, Z., Mao, X., Luo, L. (2019) Germline Stem Cells Drive Ovary Regeneration in Zebrafish. Cell Reports. 26:1709-1717.e3.
Abstract
Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping