PUBLICATION
Green Synthesis of Fluorescent Carbon Dots from Gynostemma for Bioimaging and Antioxidant in Zebrafish
- Authors
- Wei, X., Li, L., Liu, J., Yu, L., Li, H., Cheng, F., Yi, X., He, J., Li, B.
- ID
- ZDB-PUB-190214-7
- Date
- 2019
- Source
- ACS applied materials & interfaces 11(10): 9832-9840 (Journal)
- Registered Authors
- Li, Li, Liu, Jinlong
- Keywords
- none
- MeSH Terms
-
- Animals
- Antioxidants/chemistry
- Antioxidants/isolation & purification*
- Carbon/chemistry*
- Cell Survival/drug effects
- Fluorescent Dyes/chemistry
- Green Chemistry Technology
- Gynostemma/chemistry*
- Molecular Imaging/methods*
- Nitrogen/chemistry
- Quantum Dots/chemistry
- Ultraviolet Rays
- Zebrafish
- PubMed
- 30758177 Full text @ ACS Appl. Mater. Interfaces
Citation
Wei, X., Li, L., Liu, J., Yu, L., Li, H., Cheng, F., Yi, X., He, J., Li, B. (2019) Green Synthesis of Fluorescent Carbon Dots from Gynostemma for Bioimaging and Antioxidant in Zebrafish. ACS applied materials & interfaces. 11(10):9832-9840.
Abstract
Fluorescent carbon dots (CDs) have been synthesized by a calcination method using natural gynostemma as precursor, without any toxic ingredients or surface passivation chemicals. The obtained CDs have a narrow size distribution and the average particle diameter is about 2.5 nm. The CDs exhibit good water dispersibility and emits intense blue fluorescence under 360 nm UV light in an aqueous solution, which can be stable in different conditions. The biotoxicity of CDs on the embryonic development of zebrafish are evaluated, the hatch rate and the survival rate of embryos are around 90%, the malformation rate is less than 10%. Due to the excellent fluorescence stability and biocompatibility, CDs can be used in zebrafish for bioimaging. And the anti-oxidative stress property of CDs is investigated both in vitro and in vivo, the presence of CDs can promote the mRNA expression of related genes to encode more antioxidant proteins in zebrafish. Therefore, the fluorescent CDs would be a potential candidate for bioimaging and treating diseases caused by excessive oxidation damage, such as cancer, senility and other diseases associated with aging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping