ZFIN ID: ZDB-PUB-190209-18
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
Tabor, K.M., Marquart, G.D., Hurt, C., Smith, T.S., Geoca, A.K., Bhandiwad, A.A., Subedi, A., Sinclair, J.L., Rose, H.M., Polys, N.F., Burgess, H.A.
Date: 2019
Source: eLIFE   8: (Journal)
Registered Authors: Burgess, Harold, Marquart, Gregory, Subedi, Abhi, Tabor, Kathryn
Keywords: neuroscience, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • Brain/physiology
  • Brain/ultrastructure*
  • Brain Mapping/methods*
  • Cell Lineage/genetics
  • DNA-Binding Proteins/genetics
  • Gene Expression Regulation/genetics
  • Integrases/genetics
  • Neuroimaging/methods*
  • Neurons/physiology
  • Neurons/ultrastructure*
  • Transcription Factors/genetics
  • Zebrafish/genetics
  • Zebrafish/physiology
PubMed: 30735129 Full text @ Elife
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
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