PUBLICATION
Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation
- Authors
- Mehjabin, R., Chen, L., Huang, R., Zhu, D., Yang, C., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y.
- ID
- ZDB-PUB-190205-12
- Date
- 2019
- Source
- Fish & shellfish immunology 87: 788-795 (Journal)
- Registered Authors
- Zhu, Zuoyan
- Keywords
- Grass carp, Grass carp reovirus (GCRV), Host immune defences, Protein kinase C (PKC)
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/genetics*
- Carps/immunology*
- Fish Diseases/immunology*
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Gene Expression Profiling/veterinary
- Gene Expression Regulation/immunology*
- Immunity, Innate/genetics*
- Protein Kinase C-theta/chemistry
- Protein Kinase C-theta/genetics*
- Protein Kinase C-theta/immunology*
- Random Allocation
- Reoviridae/physiology
- Reoviridae Infections/immunology
- Reoviridae Infections/veterinary
- Sequence Alignment/veterinary
- PubMed
- 30716520 Full text @ Fish Shellfish Immunol.
Citation
Mehjabin, R., Chen, L., Huang, R., Zhu, D., Yang, C., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y. (2019) Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation. Fish & shellfish immunology. 87:788-795.
Abstract
Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-θ has been reported. To address this issue, we cloned the grass carp pkc-θ gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-θ was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-θ expression in the gills and spleen. Gene products that function upstream and downstream of pkc-θ were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-θ by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-θ from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-θ, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-θ in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping