PUBLICATION
Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo
- Authors
- Jia, P.P., Sun, T., Junaid, M., Yang, L., Ma, Y.B., Cui, Z.S., Wei, D.P., Shi, H.F., Pei, D.S.
- ID
- ZDB-PUB-190203-9
- Date
- 2019
- Source
- Environmental pollution (Barking, Essex : 1987) 247: 595-606 (Journal)
- Registered Authors
- Jia, Panpan, Junaid, Muhammad, Ma, Yanbo, Pei, Desheng
- Keywords
- Anti-bacterial property, Cytotoxicity, Graphene, Graphene oxide, Zebrafish
- MeSH Terms
-
- Cell Survival/drug effects
- DNA Damage
- Graphite/toxicity*
- HMGB Proteins
- Humans
- Nanostructures/toxicity*
- Oxidation-Reduction
- Oxides/chemistry*
- PubMed
- 30708322 Full text @ Environ. Pollut.
Citation
Jia, P.P., Sun, T., Junaid, M., Yang, L., Ma, Y.B., Cui, Z.S., Wei, D.P., Shi, H.F., Pei, D.S. (2019) Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo. Environmental pollution (Barking, Essex : 1987). 247:595-606.
Abstract
Graphene family nanomaterials (GFNs) have attracted significant attention due to their unique characteristics and applications in the fields of biomedicine and nanotechnology. However, previous studies highlighted the in vitro and in vivo toxicity of GFNs with size and oxidation state differences are still elusive. Therefore, we prepared graphene (G) and graphene oxide (GO) of three different sizes (S-small, M-medium, and L-large), and characterized them using multiple surface-sensitive analytical techniques. In vitro assays using HEK 293T cells revealed that the small and large sizes of G and GO significantly reduced the cell viability and increased DNA damage, accompanying with activated reactive oxygen species (ROS) generation and induced various expressions of associated critical genetic markers. Moreover, the bacterial assays highlighted that G and GO caused strong acute toxicity on Tox2 bacteria. Effects of G were higher than GO and showed size dependent effect: L > M > S, while the medium size of GO induced mild genetic toxicity on RecA bacteria. In vivo assays revealed that exposure to G and GO caused the developmental toxicity, induced ROS generation, and activated related pathways (specifically GO) in zebrafish. Taken together, G showed stronger ability to decrease the survival rate and induce the acute toxicity, while GO showed obvious toxicity in terms of DNA damages, ROS generation, and abnormal gene expressions. Our findings highlighted that G and GO differentially induced toxicity based on their varying physical characteristics, especially sizes and oxidation state, and exposure concentrations and sensitivity of the employed in vitro and in vivo models. In short, this study provided deep insights on the negative effects of GFNs exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping