PUBLICATION
ACTN2 mutations cause "Multiple structured Core Disease" (MsCD)
- Authors
- Lornage, X., Romero, N.B., Grosgogeat, C.A., Malfatti, E., Donkervoort, S., Marchetti, M.M., Neuhaus, S.B., Foley, A.R., Labasse, C., Schneider, R., Carlier, R.Y., Chao, K.R., Medne, L., Deleuze, J.F., Orlikowski, D., Bönnemann, C.G., Gupta, V.A., Fardeau, M., Böhm, J., Laporte, J.
- ID
- ZDB-PUB-190201-6
- Date
- 2019
- Source
- Acta Neuropathologica 137(3): 501-519 (Journal)
- Registered Authors
- Gupta, Vandana A
- Keywords
- ACTN2, Alpha-actinin-2, Congenital myopathy, Core myopathy, Nemaline myopathy, Z-line
- MeSH Terms
-
- Actinin/genetics*
- Animals
- Female
- Humans
- Male
- Mice
- Muscle, Skeletal/pathology*
- Mutation
- Myotonia Congenita/genetics*
- Myotonia Congenita/pathology*
- Zebrafish
- PubMed
- 30701273 Full text @ Acta Neuropathol.
Citation
Lornage, X., Romero, N.B., Grosgogeat, C.A., Malfatti, E., Donkervoort, S., Marchetti, M.M., Neuhaus, S.B., Foley, A.R., Labasse, C., Schneider, R., Carlier, R.Y., Chao, K.R., Medne, L., Deleuze, J.F., Orlikowski, D., Bönnemann, C.G., Gupta, V.A., Fardeau, M., Böhm, J., Laporte, J. (2019) ACTN2 mutations cause "Multiple structured Core Disease" (MsCD). Acta Neuropathologica. 137(3):501-519.
Abstract
The identification of genes implicated in myopathies is essential for diagnosis and for revealing novel therapeutic targets. Here we characterize a novel subclass of congenital myopathy at the morphological, molecular, and functional level. Through exome sequencing, we identified de novo ACTN2 mutations, a missense and a deletion, in two unrelated patients presenting with progressive early-onset muscle weakness and respiratory involvement. Morphological and ultrastructural analyses of muscle biopsies revealed a distinctive pattern with the presence of muscle fibers containing small structured cores and jagged Z-lines. Deeper analysis of the missense mutation revealed mutant alpha-actinin-2 properly localized to the Z-line in differentiating myotubes and its level was not altered in muscle biopsy. Modelling of the disease in zebrafish and mice by exogenous expression of mutated alpha-actinin-2 recapitulated the abnormal muscle function and structure seen in the patients. Motor deficits were noted in zebrafish, and muscle force was impaired in isolated muscles from AAV-transduced mice. In both models, sarcomeric disorganization was evident, while expression of wild-type alpha-actinin-2 did not result in muscle anomalies. The murine muscles injected with mutant ACTN2 displayed cores and Z-line defects. Dominant ACTN2 mutations were previously associated with cardiomyopathies, and our data demonstrate that specific mutations in the well-known Z-line regulator alpha-actinin-2 can cause a skeletal muscle disorder.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping