A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis
- Shi, Y., Duan, X., Xu, G., Wang, X., Wei, G., Dong, S., Xie, G., Liu, D.
- Angiogenesis 22(2): 211-221 (Journal)
- Registered Authors
- Liu, Dong, Shi, Yunwei
- Angiogenesis, Endothelial cells, MiRNA, Ribosomal DNA, Zebrafish
- MeSH Terms
- Animals, Genetically Modified
- Cloning, Molecular
- DNA, Ribosomal/genetics*
- Embryo, Nonmammalian/blood supply
- Embryonic Development/genetics
- Endothelium, Vascular/embryology*
- Endothelium, Vascular/physiology
- Neovascularization, Physiologic/genetics*
- 30656567 Full text @ Angiogenesis
Shi, Y., Duan, X., Xu, G., Wang, X., Wei, G., Dong, S., Xie, G., Liu, D. (2019) A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis. Angiogenesis. 22(2):211-221.
MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18-25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes