PUBLICATION
The Surface Proteome of Adult Neural Stem Cells in Zebrafish Unveils Long-Range Cell-Cell Connections and Age-Related Changes in Responsiveness to IGF
- Authors
- Obermann, J., Wagner, F., Kociaj, A., Zambusi, A., Ninkovic, J., Hauck, S.M., Chapouton, P.
- ID
- ZDB-PUB-190115-3
- Date
- 2019
- Source
- Stem Cell Reports 12(2): 258-273 (Journal)
- Registered Authors
- Chapouton, Prisca, Ninkovic, Jovica
- Keywords
- GFAP, aging, biotinylation, filopodia, lamellipodia, mass spectrometry, neurogenesis, pallium, quiescence, radial glia, telencephalon
- MeSH Terms
-
- Adult Stem Cells/metabolism*
- Adult Stem Cells/physiology
- Animals
- Brain/metabolism
- Brain/physiology
- Cell Cycle/physiology
- Cell Differentiation/physiology
- Cell Division/physiology
- Cell Proliferation/physiology
- Neural Stem Cells/metabolism
- Neural Stem Cells/physiology
- Neurogenesis/physiology
- Proteome/metabolism*
- Signal Transduction/physiology
- Somatomedins/metabolism*
- Telencephalon/metabolism
- Telencephalon/physiology
- Zebrafish
- PubMed
- 30639211 Full text @ Stem Cell Reports
Citation
Obermann, J., Wagner, F., Kociaj, A., Zambusi, A., Ninkovic, J., Hauck, S.M., Chapouton, P. (2019) The Surface Proteome of Adult Neural Stem Cells in Zebrafish Unveils Long-Range Cell-Cell Connections and Age-Related Changes in Responsiveness to IGF. Stem Cell Reports. 12(2):258-273.
Abstract
In adult stem cell populations, recruitment into division is parsimonious and most cells maintain a quiescent state. How individual cells decide to enter the cell cycle and how they coordinate their activity remains an essential problem to be resolved. It is thus important to develop methods to elucidate the mechanisms of cell communication and recruitment into the cell cycle. We made use of the advantageous architecture of the adult zebrafish telencephalon to isolate the surface proteins of an intact neural stem cell (NSC) population. We identified the proteome of NSCs in young and old brains. The data revealed a group of proteins involved in filopodia, which we validated by a morphological analysis of single cells, showing apically located cellular extensions. We further identified an age-related decrease in insulin-like growth factor (IGF) receptors. Expressing IGF2b induced divisions in young brains but resulted in incomplete divisions in old brains, stressing the role of cell-intrinsic processes in stem cell behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping