PUBLICATION
Nonpathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish
- Authors
- Schulz, A., Brendler, J., Blaschuk, O., Landgraf, K., Krueger, M., Ricken, A.M.
- ID
- ZDB-PUB-190109-12
- Date
- 2019
- Source
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 67(5): 361-373 (Journal)
- Registered Authors
- Keywords
- atrioventricular valve, bulboventricular valve, cartilage, chondroblast, chondrogenesis, teleost
- MeSH Terms
-
- Aging*
- Animals
- Cartilage/cytology
- Chondrogenesis*
- Collagen/analysis
- Heart Valves/cytology*
- Heart Valves/growth & development
- Heart Valves/pathology
- Heart Valves/ultrastructure*
- Mice
- Mice, Inbred C57BL
- Species Specificity
- Zebrafish
- PubMed
- 30620237 Full text @ J. Histochem. Cytochem.
Citation
Schulz, A., Brendler, J., Blaschuk, O., Landgraf, K., Krueger, M., Ricken, A.M. (2019) Nonpathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 67(5):361-373.
Abstract
In the heart, unidirectional blood flow depends on proper heart valve function. As, in mammals, regulatory mechanisms of early heart valve and bone development are shown to contribute to adult heart valve pathologies, we used the animal model zebrafish (ZF, Danio rerio) to investigate the microarchitecture and differentiation of cardiac valve interstitial cells in the transition from juvenile (35 days) to end of adult breeding (2.5 years) stages. Of note, light microscopy and immunohistochemistry revealed major differences in ZF heart valve microarchitecture when compared with adult mice. We demonstrate evidence for rather chondrogenic features of valvular interstitial cells by histological staining and immunodetection of SOX-9, aggrecan, and type 2a1 collagen. Collagen depositions are enriched in a thin layer at the atrial aspect of atrioventricular valves and the ventricular aspect of bulboventricular valves, respectively. At the ultrastructural level, the collagen fibrils are lacking obvious periodicity and orientation throughout the entire valve.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping