PUBLICATION
Fluorescently Labeled TracrRNA Improves Work Flow and Facilitates Successful Genome Editing in Zebrafish
- Authors
- Hamimi, M., Khabooshan, M., Castillo, H.A., Kaslin, J.
- ID
- ZDB-PUB-181227-6
- Date
- 2018
- Source
- Zebrafish 16(1): 135-137 (Other)
- Registered Authors
- Kaslin, Jan
- Keywords
- CRISPR, knockin, knockout, loss-of-function, mutant, teleost
- MeSH Terms
-
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats*
- Gene Editing/instrumentation
- Gene Editing/methods*
- Genome*
- Heterocyclic Compounds, 4 or More Rings/chemistry*
- Zebrafish/genetics*
- PubMed
- 30585775 Full text @ Zebrafish
Citation
Hamimi, M., Khabooshan, M., Castillo, H.A., Kaslin, J. (2018) Fluorescently Labeled TracrRNA Improves Work Flow and Facilitates Successful Genome Editing in Zebrafish. Zebrafish. 16(1):135-137.
Abstract
Gene editing using clustered regularly interspaced short palindromic repeats (CRISPR) is widely used throughout the zebrafish community for the generation of knockouts and knockins. One of the bottlenecks that exists during the process is the laborious screening of injected embryos for F0 founder fish or CRISPants, weeks after the injection date. In this study we show that the use of fluorescently tagged tracrRNA and sorting for fluorescent embryos as early as the 512-cell stage using stereomicroscope significantly improve yield of fish with successfully CRISPR/Cas9-edited genomes. This is a cost-effective strategy that significantly improves workflow and efficacy in genome editing in particular for less experienced researchers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping