PUBLICATION
Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model
- Authors
- Liu, M., Copmans, D., Lu, J.G., Yang, M.R., Sourbron, J., Ny, A., Jiang, Z.H., de Witte, P.A.M., Luyten, W.
- ID
- ZDB-PUB-181221-14
- Date
- 2018
- Source
- Journal of ethnopharmacology 232: 130-134 (Journal)
- Registered Authors
- Keywords
- Semen Pharbitidis, anti-seizure, pharbitin, zebrafish
- MeSH Terms
-
- Animals
- Anticonvulsants/therapeutic use*
- Butyrates/therapeutic use
- Glycosides/therapeutic use*
- Ipomoea nil*
- Pentylenetetrazole
- Plant Extracts/therapeutic use*
- Resins, Plant/therapeutic use*
- Seeds
- Seizures/chemically induced
- Seizures/drug therapy*
- Zebrafish
- PubMed
- 30572093 Full text @ J. Ethnopharmacol.
Citation
Liu, M., Copmans, D., Lu, J.G., Yang, M.R., Sourbron, J., Ny, A., Jiang, Z.H., de Witte, P.A.M., Luyten, W. (2018) Bioassay-guided isolation of anti-seizure principles from Semen Pharbitidis using a zebrafish pentylenetetrazol seizure model. Journal of ethnopharmacology. 232:130-134.
Abstract
Ethnopharmacological relevance Semen Pharbitidis, the seeds of Pharbitis nil (Linn.) Choisy (Convolvulaceae) is a well-known traditional Chinese medicinal plant used for treating helminthiasis and epilepsy in China.
Aim of the study This study aims to identify the anti-seizure components from Semen Pharbitidis.
Methods A bioassay-guided isolation of anti-seizure compounds from Semen Pharbitidis was performed using a zebrafish pentylenetetrazol seizure model. The structures of active compounds were elucidated by high resolution mass spectrometry. The fragments of active compounds were tested for anti-seizure activity as well.
Results The bioassay-guided isolation of ethanol extract of Semen Pharbitidis led to a group of resin glucosides, namely pharbitin. One of the fragments of pharbitin, 2-methylbutyric acid, also showed anti-seizure activity.
Conclusions We provided further experimental scientific evidence to support the traditional use of Semen Pharbitidis for the treatment of epilepsy. Pharbitin was identified to be the main anti-seizure component in Semen Pharbitidis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping