PUBLICATION
Agaricus bisporus-derived β-glucan prevents obesity through PPAR γ downregulation and autophagy induction in zebrafish fed by chicken egg yolk
- Authors
- Li, X., Xue, Y., Pang, L., Len, B., Lin, Z., Huang, J., ShangGuan, Z., Pan, Y.
- ID
- ZDB-PUB-181218-6
- Date
- 2018
- Source
- International journal of biological macromolecules 125: 820-828 (Journal)
- Registered Authors
- Xue, Yu
- Keywords
- Autophagy, Lipid metabolism, PPAR γ, Zebrafish, β-Glucan
- MeSH Terms
-
- Adipocytes/drug effects
- Agaricus/chemistry*
- Animals
- Autophagy/drug effects*
- Chickens/metabolism
- Down-Regulation/drug effects*
- Egg Yolk/metabolism
- Obesity/metabolism*
- Obesity/prevention & control*
- PPAR gamma/metabolism*
- Zebrafish
- beta-Glucans/pharmacology*
- PubMed
- 30557642 Full text @ Int. J. Biol. Macromol.
Citation
Li, X., Xue, Y., Pang, L., Len, B., Lin, Z., Huang, J., ShangGuan, Z., Pan, Y. (2018) Agaricus bisporus-derived β-glucan prevents obesity through PPAR γ downregulation and autophagy induction in zebrafish fed by chicken egg yolk. International journal of biological macromolecules. 125:820-828.
Abstract
β-(1,4)-d-Glucan with (1,2) and (1,6)-linked branches (short for β-glucan), extracted from Agaricus bisporus (Lange) Sing, had significant anti-obesity and lowering-fat effect. FITC-β-glucan was absorbed by adipocytes of zebrafish larvae when stained by Nile Red. β-Glucan decreased the adiposity mass, reduced the expression of ppar g, mtp, L-fabp, ifabp in ISH, which was coincident as the results of RT-PCT. β-Glucan lowered the level of C/EBP α, c SREBP1, LXR α, PPAR γ by WB analysis, which were accompanied by an increase level in LC3 II/LC3 I and a decline level in p62 in dose-dependent manner. This study explored the effect and mechanisms of Agaricus bisporus derived-β-glucan to regulate lipid metabolism and prevent lipid deposits, and provided the experimental data for its use in diet food and food addictive.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping