PUBLICATION

Hif-1α-Induced Expression of Il-1β Protects against Mycobacterial Infection in Zebrafish

Authors
Ogryzko, N.V., Lewis, A., Wilson, H.L., Meijer, A.H., Renshaw, S.A., Elks, P.M.
ID
ZDB-PUB-181216-5
Date
2018
Source
Journal of immunology (Baltimore, Md. : 1950)   202(2): 494-502 (Journal)
Registered Authors
Elks, Phil, Meijer, Annemarie H., Ogryzko, Nikolay, Renshaw, Steve A.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Antitubercular Agents/metabolism
  • Cells, Cultured
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Disease Models, Animal
  • Gene Knockout Techniques
  • Green Fluorescent Proteins/genetics
  • Humans
  • Hypoxia/metabolism*
  • Hypoxia-Inducible Factor 1, alpha Subunit/genetics
  • Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
  • Immunity, Innate
  • Interleukin-1beta/genetics
  • Interleukin-1beta/metabolism*
  • Macrophages/immunology*
  • Mycobacterium Infections, Nontuberculous/immunology*
  • Mycobacterium marinum/physiology*
  • Mycobacterium tuberculosis/physiology*
  • Nitric Oxide/metabolism
  • Tuberculosis/immunology*
  • Zebrafish
PubMed
30552162 Full text @ J. Immunol.
Abstract
Drug-resistant mycobacteria are a rising problem worldwide. There is an urgent need to understand the immune response to tuberculosis to identify host targets that, if targeted therapeutically, could be used to tackle these currently untreatable infections. In this study we use an Il-1β fluorescent transgenic line to show that there is an early innate immune proinflammatory response to well-established zebrafish models of inflammation and Mycobacterium marinum infection. We demonstrate that host-derived hypoxia signaling, mediated by the Hif-1α transcription factor, can prime macrophages with increased levels of Il-1β in the absence of infection, upregulating neutrophil antimicrobial NO production, leading to greater protection against infection. Our data link Hif-1α to proinflammatory macrophage Il-1β transcription in vivo during early mycobacterial infection and importantly highlight a host protective mechanism, via antimicrobial NO, that decreases disease outcomes and that could be targeted therapeutically to stimulate the innate immune response to better deal with infections.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes