PUBLICATION
3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model
- Authors
- Ko, E.Y., Heo, S.J., Cho, S.H., Lee, W., Kim, S.Y., Yang, H.W., Ahn, G., Cha, S.H., Kwon, S.H., Jeong, M.S., Lee, K.P., Jeon, Y.J., Kim, K.N.
- ID
- ZDB-PUB-181212-26
- Date
- 2018
- Source
- International Immunopharmacology 67: 98-105 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Anti-inflammatory, BEMB, NF-κB, RAW264.7 cells, ROS, Zebrafish embryo
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/therapeutic use*
- Benzene/chemistry
- Benzene/therapeutic use*
- Disease Models, Animal
- Down-Regulation
- Embryo, Nonmammalian
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Humans
- Inflammation/drug therapy*
- Lipopolysaccharides/immunology
- Mice
- NF-kappa B/genetics
- NF-kappa B/metabolism*
- Nitric Oxide/metabolism
- Nitric Oxide Synthase Type II/genetics
- Nitric Oxide Synthase Type II/metabolism
- RAW 264.7 Cells
- Reactive Oxygen Species/metabolism
- Rhodophyta/immunology
- Zebrafish
- PubMed
- 30537636 Full text @ Int. Immunopharmacol.
Citation
Ko, E.Y., Heo, S.J., Cho, S.H., Lee, W., Kim, S.Y., Yang, H.W., Ahn, G., Cha, S.H., Kwon, S.H., Jeong, M.S., Lee, K.P., Jeon, Y.J., Kim, K.N. (2018) 3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model. International Immunopharmacology. 67:98-105.
Abstract
The anti-inflammatory effects of 3‑bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol (BEMB) from Polysiphonia morrowii were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells and zebrafish embryo. BEMB showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS), and the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in the LPS-activated RAW264.7 cells and zebrafish embryo without cytotoxicity. Moreover, BEMB suppressed the protein and mRNA expression levels of nuclear factor (NF)-κB (p65 and inhibitor of NF-κB [IκB]-A) in RAW264.7 cells and zebrafish embryo, respectively. Collectively, the results of this study indicate that BEMB suppressed the production of pro-inflammatory mediators such as NO, iNOS, and COX-2 as well as their regulation in LPS-induced RAW264.7 cells and zebrafish embryos by inhibiting ROS production and NF-κB expression. Therefore, this study suggests that BEMB could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping