PUBLICATION
A transcriptomics analysis of the Tbx5 paralogues in zebrafish
- Authors
- Boyle Anderson, E.A.T., Ho, R.K.
- ID
- ZDB-PUB-181212-15
- Date
- 2018
- Source
- PLoS One 13: e0208766 (Journal)
- Registered Authors
- Ho, Robert K.
- Keywords
- none
- Datasets
- GEO:GSE115640
- MeSH Terms
-
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Animals
- Myocardium/metabolism
- Zebrafish
- Computational Biology
- Gene Knockdown Techniques
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Gene Expression Profiling
- Eye/growth & development
- Eye/metabolism
- Transcriptome
- Gene Expression Regulation, Developmental
- Heart/growth & development
- PubMed
- 30532148 Full text @ PLoS One
Citation
Boyle Anderson, E.A.T., Ho, R.K. (2018) A transcriptomics analysis of the Tbx5 paralogues in zebrafish. PLoS One. 13:e0208766.
Abstract
TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping