PUBLICATION
Can environmentally relevant neuroactive chemicals specifically be detected with the locomotor response test in zebrafish embryos?
- Authors
- Leuthold, D., Klüver, N., Altenburger, R., Busch, W.
- ID
- ZDB-PUB-181206-12
- Date
- 2018
- Source
- Environmental science & technology 53(1): 482-493 (Journal)
- Registered Authors
- Klüver, Nils
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*
- Zebrafish*
- PubMed
- 30516976 Full text @ Env. Sci. Tech.
Citation
Leuthold, D., Klüver, N., Altenburger, R., Busch, W. (2018) Can environmentally relevant neuroactive chemicals specifically be detected with the locomotor response test in zebrafish embryos?. Environmental science & technology. 53(1):482-493.
Abstract
Chemicals considered as neuroactive (such as certain pesticides, pharmaceuticals and industrial chemicals) are among the largest groups of bioactive substances recently detected in European rivers. However, the determination of nervous system-specific effects has been limited using in vitro tests or conventional endpoints including lethality. Thus, neurobehavioral tests using in vivo models (e.g. zebrafish embryo) have been proposed as complementary approaches. To investigate the specificity and sensitivity of a light-dark transition locomotor response (LMR) test in 4 to 5 days post fertilization zebrafish with respect to different modes of action (MoAs), we analyzed a set of 18 environmentally relevant compounds with various anticipated MoAs. We found that exposure-induced behavioral alterations were reproducible and dependent on concentration and time. Comparative and quantitative analyses of the obtained locomotor patterns revealed that behavioral effects were not restricted to compounds primarily known to target the nervous system. A clear distinction of MoAs based on locomotor patterns was not possible for most compounds. Furthermore, chemicals with an anticipated same MoA did not necessarily provoke similar behavioral phenotypes. Finally, we determined an increased sensitivity (≥10-fold) compared to observed mortality in the LMR assay for 5 of 8 neuroactive chemicals as opposed to non-neuroactive compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping