ZFIN ID: ZDB-PUB-181130-18
Dynamic and non-contact 3D sample rotation for microscopy
Berndt, F., Shah, G., Power, R.M., Brugués, J., Huisken, J.
Date: 2018
Source: Nature communications   9: 5025 (Journal)
Registered Authors: Huisken, Jan
Keywords: none
MeSH Terms: none
PubMed: 30487638 Full text @ Nat. Commun.
Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.