ZFIN ID: ZDB-PUB-181127-43
Chiral Cilia Orientation in the Left-Right Organizer
Ferreira, R.R., Pakula, G., Klaeyle, L., Fukui, H., Vilfan, A., Supatto, W., Vermot, J.
Date: 2018
Source: Cell Reports   25: 2008-2016.e4 (Journal)
Registered Authors: Ferreira, Rita, Fukui, Hajime, Vermot, Julien
Keywords: Danio rerio, Dnaaf1/Lrrc50, PKD2/PC2/TRPP2/polycistin-2, Rock2B, blebbistatin, lrdr1, nodal, spaw, tissue asymmetry, trilobite/Vangl2
MeSH Terms:
  • Animals
  • Basal Bodies/metabolism
  • Body Patterning*
  • Cilia/metabolism*
  • Organizers, Embryonic/physiology
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism
PubMed: 30462999 Full text @ Cell Rep.
Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and many cell types are chiral. In the left-right organizer (LRO), cilia-driven flows transfer such chirality to the body scale. However, the existence of cellular chirality within tissues remains unknown. Here, we investigate this question in Kupffer's vesicle (KV), the zebrafish LRO. Quantitative live imaging reveals that cilia populating the KV display asymmetric orientation between the right and left sides, resulting in a chiral structure, which is different from the chiral cilia rotation. This KV chirality establishment is dynamic and depends on planar cell polarity. While its impact on left-right (LR) symmetry breaking remains unclear, we show that this asymmetry does not depend on the LR signaling pathway or flow. This work identifies a different type of tissue asymmetry and sheds light on chirality genesis in developing tissues.