PUBLICATION

Pseudocapillaria tomentosa in laboratory zebrafish Danio rerio: patterns of infection and dose response

Authors
Kent, M.L., Gaulke, C.A., Watral, V., Sharpton, T.J.
ID
ZDB-PUB-181127-38
Date
2018
Source
Diseases of aquatic organisms   131: 121-131 (Journal)
Registered Authors
Kent, Michael
Keywords
Experimental infection, Nematoda, Parasitism, Pseudocapillaria, Zebrafish
MeSH Terms
  • Animals
  • Female
  • Fish Diseases/parasitology*
  • Laboratory Animal Science*
  • Male
  • Nematoda/classification*
  • Nematode Infections/parasitology
  • Nematode Infections/veterinary*
  • Zebrafish*
PubMed
30460918 Full text @ Dis. Aquat. Organ.
Abstract
Parasites in wild populations almost always exhibit aggregation (overdispersion), in which relatively few hosts are infected with high numbers of the parasites. This pattern of infection has also been observed in laboratory studies, where many of the sources of natural variation are removed. Pseudocapillaria tomentosa (Nematoda) is common in zebrafish (Danio rerio) facilities. We describe here patterns of infections in zebrafish experimentally infected with larvated P. tomentosa eggs in various trials with defined numbers of eggs. One trial with eggs delivered in a gelatin diet is also included. Fish were exposed at 25, 75, and 200 eggs fish-1, and the minimal infectious dose was estimated to be 1.5 eggs fish-1. The ID50 (50% infective dose) was calculated to be 17.5 eggs fish-1. We also included data from a trial and 2 previously published experiments with undefined doses in which zebrafish were exposed to infectious water and detritus from a tank that previously contained infected fish. All doses resulted in a high prevalence of infection (>70%), except at the 25 eggs fish-1 dose, where the prevalence was 43-46%. Mean abundance of worms corresponded to dose, from 0.57 worms fish-1 at 25 eggs fish-1 to 7 worms fish-1 at 200 eggs fish-1. Variance to mean ratios (V/M) and the k parameters showed aggregation across the 8 separate trials, including the gelatin diet. Aggregation increased with increased parasite abundance. Given the consistent observation of aggregation across our experiments, the zebrafish/P. tomentosa system provides a potentially robust, high-throughput model to investigate factors that influence differences in host susceptibility within defined populations.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes