|ZFIN ID: ZDB-PUB-181118-16|
Identification of novel circadian transcripts in the zebrafish retina
Ramasamy, S., Sharma, S., Iyengar, B.R., Vellarikkal, S.K., Sivasubbu, S., Maiti, S., Pillai, B.
|Source:||The Journal of experimental biology 222(Pt 1): (Journal)|
|Registered Authors:||Pillai, Beena, Sivasubbu, Sridhar|
|Keywords:||Circadian rhythms, RNA-seq, Retina, Transcriptomics, Zebrafish|
|PubMed:||30446534 Full text @ J. Exp. Biol.|
Ramasamy, S., Sharma, S., Iyengar, B.R., Vellarikkal, S.K., Sivasubbu, S., Maiti, S., Pillai, B. (2018) Identification of novel circadian transcripts in the zebrafish retina. The Journal of experimental biology. 222(Pt 1):.
ABSTRACTHigh fecundity, transparent embryos for monitoring the rapid development of organs and the availability of a well-annotated genome has made zebrafish a model organism of choice for developmental biology and neurobiology. This vertebrate model, a favourite in chronobiology studies, shows striking circadian rhythmicity in behaviour. Here, we identify novel genes in the zebrafish genome, which are expressed in the zebrafish retina. We further resolve the expression pattern over time and tentatively assign specific novel transcripts to retinal bipolar cells of the inner nuclear layer. Using chemical ablation and free run experiments we segregate the transcripts that are rhythmic when entrained by light from those that show sustained oscillations in the absence of external cues. The transcripts reported here with rigorous annotation and specific functions in circadian biology provide the groundwork for functional characterisation of novel players in the zebrafish retinal clock.
- Genes / Markers (7)