PUBLICATION
Characterization and functional analysis of grouper (Epinephelus coioides) MEK1 and MEK2
- Authors
- Mo, Z.Q., Han, R., Wang, J.L., Ni, L.Y., Su, Y.L., Lai, X.L., He, Z.C., Chen, H.P., Li, Y.W., Sun, H.Y., Luo, X.C., Dan, X.M.
- ID
- ZDB-PUB-181114-21
- Date
- 2018
- Source
- Fish & shellfish immunology 84: 1090-1097 (Journal)
- Registered Authors
- Keywords
- AP1, Cryptocaryon irritans, ERK1/2, Epinephelus coioides, MEK1/2
- MeSH Terms
-
- Bass/genetics*
- Bass/immunology*
- Animals
- Immunity, Innate/genetics*
- Phylogeny
- Amino Acid Sequence
- Fish Diseases/immunology*
- Gene Expression Regulation/immunology*
- Sequence Alignment/veterinary
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/immunology*
- MAP Kinase Kinase 1/genetics
- MAP Kinase Kinase 1/immunology
- MAP Kinase Kinase 2/genetics
- MAP Kinase Kinase 2/immunology
- Gene Expression Profiling/veterinary
- PubMed
- 30419398 Full text @ Fish Shellfish Immunol.
Citation
Mo, Z.Q., Han, R., Wang, J.L., Ni, L.Y., Su, Y.L., Lai, X.L., He, Z.C., Chen, H.P., Li, Y.W., Sun, H.Y., Luo, X.C., Dan, X.M. (2018) Characterization and functional analysis of grouper (Epinephelus coioides) MEK1 and MEK2. Fish & shellfish immunology. 84:1090-1097.
Abstract
MEK dual-specificity protein kinases are a group of mitogen-activated protein kinase kinases, which act as an integration point by transferring extracellular signals to the nucleus. To investigate the function of MEK in teleost fish, we cloned MEK1 and MEK2 cDNA sequences from the orange-spotted grouper (Epinephelus coioides). EcMEK1 and EcMEK2 shared 80% amino acid identity with each other. EcMEK1 had 89-99% amino acid identity with teleosts or mammals, whereas EcMEK2 shared 85-97% amino acid identity. The exon structures of the grouper MEK1/2 genes were conserved with zebrafish and human MEK1/2. Tissue distribution analysis showed that EcMEK1 and EcMEK2 had a similar expression pattern in grouper tissues and was mainly transcribe in systemic immune organs. Both EcMEK1 and EcMEK2 were distributed throughout the cytoplasm of transfected GS or HEK293T cells. Overexpression of EcMEK1 or EcMEK2 activated Activator protein 1 dependent luciferase. The phosphorylation levels of EcMEK1/2 and EcERK1/2 were significantly increased in head kidney leukocytes by stimulation with PMA treatment. The grouper MEK1/2-ERK1/2 axis was activated in Cryptocaryon irritans infection and showed an enhanced phosphorylation after immunization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping