PUBLICATION
Effects of lidocaine on adult zebrafish behavior and brain acetylcholinesterase following peripheral and systemic administration
- Authors
- de Abreu, M.S., Giacomini, A.C.V.V., Dos Santos, B.E., Genario, R., Marchiori, N.I., Rosa, L.G.D., Kalueff, A.V.
- ID
- ZDB-PUB-181107-15
- Date
- 2018
- Source
- Neuroscience letters 692: 181-186 (Journal)
- Registered Authors
- Kalueff, Allan V.
- Keywords
- anxiety, emotional behavior, lidocaine, sedation, zebrafish
- MeSH Terms
-
- Acetylcholinesterase/metabolism*
- Animals
- Anxiety/chemically induced
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Brain/enzymology
- Exploratory Behavior/drug effects
- Female
- Lidocaine/administration & dosage*
- Male
- Models, Animal
- Swimming
- Zebrafish
- PubMed
- 30399397 Full text @ Neurosci. Lett.
Citation
de Abreu, M.S., Giacomini, A.C.V.V., Dos Santos, B.E., Genario, R., Marchiori, N.I., Rosa, L.G.D., Kalueff, A.V. (2018) Effects of lidocaine on adult zebrafish behavior and brain acetylcholinesterase following peripheral and systemic administration. Neuroscience letters. 692:181-186.
Abstract
Lidocaine is a voltage-gated Na+ channel blocker, commonly used as a fast-acting local and general anesthetic. Lidocaine also has central action, and affects behavior both clinically and in animal models. Adult zebrafish are rapidly becoming a critical novel model organism in translational neuroscience research. Here, we examine the effects of peripheral (lateral line application, 4%) and systemic (water immersion, 1, 5 and 10 mg/L) administration of lidocaine on adult zebrafish behavior tested in the novel tank test. Overall, the drug evoked hypolocomotor effect applied systemically (at 10 mg/L) and peripherally. This study shows the importance of the lateral line system in driving adult zebrafish locomotion, and suggests sedative-like effects of systemic lidocaine in aduld zebrafish. In addition, reflecting the role of central cholinergic contribution in lidocaine action, brain acetylcholinesterase (AChE) activity was lower following peripheral and systemic administration of lidocaine at behaviorally active doses. Collectively, our data support lidocaine effects on behavioral responses in zebrafish, and reinforce the growing utility of this aquatic model to screen CNS drugs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping