PUBLICATION
Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue
- Authors
- Geurtzen, K., Knopf, F.
- ID
- ZDB-PUB-181106-26
- Date
- 2018
- Source
- Journal of visualized experiments : JoVE (140): (Other)
- Registered Authors
- Knopf, Franziska
- Keywords
- none
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Prednisolone/pharmacology
- Prednisolone/therapeutic use*
- Regeneration/drug effects*
- Zebrafish
- PubMed
- 30394396 Full text @ J. Vis. Exp.
Citation
Geurtzen, K., Knopf, F. (2018) Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue. Journal of visualized experiments : JoVE. (140).
Abstract
Zebrafish are able to regenerate various organs, including appendages (fins) after amputation. This involves the regeneration of bone, which regrows within roughly two weeks after injury. Furthermore, zebrafish are able to heal bone rapidly after trepanation of the skull, and repair fractures that can be easily introduced into zebrafish bony fin rays. These injury assays represent feasible experimental paradigms to test the effect of administered drugs on rapidly forming bone. Here, we describe the use of these 3 injury models and their combined use with systemic glucocorticoid treatment, which exerts bone inhibitory and immunosuppressive effects. We provide a workflow on how to prepare for immunosuppressive treatment in adult zebrafish, illustrate how to perform fin amputation, trepanation of calvarial bones, and fin fractures, and describe how the use of glucocorticoids affects both bone forming osteoblasts and cells of the monocyte/macrophage lineage as part of innate immunity in bone tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping