PUBLICATION
Visualizing Photodynamic Therapy in Transgenic Zebrafish Using Organic Nanoparticles with Aggregation-Induced Emission
- Authors
- Manghnani, P.N., Wu, W., Xu, S., Hu, F., Teh, C., Liu, B.
- ID
- ZDB-PUB-181106-24
- Date
- 2018
- Source
- Nano-micro letters 10: 61 (Journal)
- Registered Authors
- Teh, Cathleen
- Keywords
- Aggregation-induced emission, Nanomedicine, Organic nanoparticles, Photodynamic therapy, Transgenic zebrafish
- MeSH Terms
- none
- PubMed
- 30393709 Full text @ Nanomicro Lett
Citation
Manghnani, P.N., Wu, W., Xu, S., Hu, F., Teh, C., Liu, B. (2018) Visualizing Photodynamic Therapy in Transgenic Zebrafish Using Organic Nanoparticles with Aggregation-Induced Emission. Nano-micro letters. 10:61.
Abstract
Photodynamic therapy (PDT) employs accumulation of photosensitizers (PSs) in malignant tumor tissue followed by the light-induced generation of cytotoxic reactive oxygen species to kill the tumor cells. The success of PDT depends on optimal PS dosage that is matched with the ideal power of light. This in turn depends on PS accumulation in target tissue and light administration time and period. As theranostic nanomedicine is driven by multifunctional therapeutics that aim to achieve targeted tissue delivery and image-guided therapy, fluorescent PS nanoparticle (NP) accumulation in target tissues can be ascertained through fluorescence imaging to optimize the light dose and administration parameters. In this regard, zebrafish larvae provide a unique transparent in vivo platform to monitor fluorescent PS bio-distribution and their therapeutic efficiency. Using fluorescent PS NPs with unique aggregation-induced emission characteristics, we demonstrate for the first time the real-time visualization of polymeric NP accumulation in tumor tissue and, more importantly, the best time to conduct PDT using transgenic zebrafish larvae with inducible liver hyperplasia as an example.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping