PUBLICATION
Generation of a Tg(cyp1a-12DRE:EGFP) transgenic zebrafish line as a rapid in vivo model for detecting dioxin-like compounds
- Authors
- Shen, C., Zhou, Y., Ruan, J., Chuang, Y.J., Wang, C., Zuo, Z.
- ID
- ZDB-PUB-181106-14
- Date
- 2018
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 205: 174-181 (Journal)
- Registered Authors
- Chao, Shen, Chuang, Yung-Jen, He, Chengyong, Jinpeng, Ruan, Yixi, Zhou, Zhenghong, Zuo
- Keywords
- DLCs, Danio rerio, TCDD, Transgenic fish, cyp1a promoter
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biological Assay/methods*
- Cell Line
- Dioxins/analysis*
- Dioxins/metabolism
- Environmental Monitoring/methods*
- Water Pollutants, Chemical/toxicity
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 30391726 Full text @ Aquat. Toxicol.
Citation
Shen, C., Zhou, Y., Ruan, J., Chuang, Y.J., Wang, C., Zuo, Z. (2018) Generation of a Tg(cyp1a-12DRE:EGFP) transgenic zebrafish line as a rapid in vivo model for detecting dioxin-like compounds. Aquatic toxicology (Amsterdam, Netherlands). 205:174-181.
Abstract
Dioxin-like compounds (DLCs) are extremely stable toxic organic compounds and can cause serious health risks. To develop a convenient biomonitoring tool for the detection of DLCs in the environment, we generated a transgenic line-Tg(cyp1a-12DRE:EGFP)-with a zebrafish cyp1a promoter recombined with multiple dioxin-responsive elements (DREs) that drive EGFP expression. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced EGFP expression was observed in the head cartilage (most sensitive), gut, otic vesicle, pectoral fin bud and eye of larvae. The lowest observed effect concentration of TCDD was estimated to be approximately 1 ng/L. Compared with existing zebrafish lines, our transgenic fish displayed comparable or even higher detection sensitivity to DLCs and could serve as an improved and rapid assay in an in vivo context. The Tg(cyp1a-12DRE:EGFP) transgenic zebrafish line also had higher stability for inducing EGFP expression (nearly 100% of our zebrafish induced EGFP at approximately 1 ng/L TCDD) than other lines. In addition, Tg(cyp1a-12DRE:EGFP) zebrafish could serve as a convenient and straightforward tool to assess potential cranial malformations and related health effects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping