PUBLICATION
Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab
- Authors
- Liao, M.L., Peng, W.H., Kan, D., Chien, C.L.
- ID
- ZDB-PUB-181103-5
- Date
- 2018
- Source
- Journal of neuroscience research 97(2): 202-214 (Journal)
- Registered Authors
- Keywords
- development, neurofilament, pineal gland, zebrafish (RRID:ZIRC_ZL1), α-internexin
- MeSH Terms
-
- Antibody Formation
- Zebrafish Proteins/metabolism*
- Intermediate Filament Proteins/metabolism*
- Pineal Gland/metabolism
- Animals
- Gene Expression Regulation, Developmental
- Retina/metabolism
- Zebrafish/metabolism*
- PubMed
- 30387501 Full text @ J. Neurosci. Res.
Citation
Liao, M.L., Peng, W.H., Kan, D., Chien, C.L. (2018) Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab. Journal of neuroscience research. 97(2):202-214.
Abstract
It has been reported that the neuronal intermediate filament (IF) α-internexin may plays a role in the formation of the neuronal cytoskeleton during mammalian development. From a phylogenetic viewpoint, zebrafish express inaa and inab as homologs of mammalian α-internexin. However, the distribution patterns of the inaa and inab proteins throughout zebrafish development have not been well-characterized. We generated antibodies specific for zebrafish inaa and inab and analyzed the distribution of these two proteins in developing zebrafish. Inaa was identified in the major subdivisions of embryonic and larval brains as early as 1 day postfertilization (dpf), including the telencephalon, optic tectum, and cerebellum, and inab was also detected in the same regions from 3 dpf to the adult stage. Moreover, we demonstrated for the first time that inaa was distinctively expressed in the photoreceptor-like cells of the pineal gland, where inab was sparsely detected. Besides, the expression of inaa in male adult fish was found to be stable under different photoperiod conditions. Thus, we suggest that inaa is one of useful markers for studies of zebrafish cone photoreceptors not only in the retina but also in the pineal gland. In conclusion, we report that the distribution patterns of inaa and inab are phylogenetically conserved in the telencephalon, optic tectum, and cerebellum. Moreover, inaa and inab had different expression patterns in the pineal gland and retina during zebrafish development. Both inaa and inab are neuronal IFs and their functional roles may be different in various aspects of zebrafish neuronal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping