PUBLICATION
Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish
- Authors
- Lin, M.H., Chou, H.C., Chen, Y.F., Liu, W., Lee, C.C., Liu, L.Y., Chuang, Y.J.
- ID
- ZDB-PUB-181031-7
- Date
- 2018
- Source
- Scientific Reports 8: 15986 (Journal)
- Registered Authors
- Chuang, Yung-Jen
- Keywords
- none
- MeSH Terms
-
- Animals
- Cardiac Electrophysiology/methods
- Cardiovascular System/diagnostic imaging
- Cardiovascular System/drug effects
- Disease Models, Animal
- Drug Evaluation, Preclinical*
- Electrocardiography/instrumentation*
- Electrocardiography/methods
- Heart/diagnostic imaging
- Heart/drug effects*
- Heart Diseases/diagnostic imaging
- Heart Diseases/drug therapy*
- Humans
- Zebrafish/physiology
- PubMed
- 30375400 Full text @ Sci. Rep.
Citation
Lin, M.H., Chou, H.C., Chen, Y.F., Liu, W., Lee, C.C., Liu, L.Y., Chuang, Y.J. (2018) Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish. Scientific Reports. 8:15986.
Abstract
Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping