PUBLICATION
Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis
- Authors
- de Pater, E., Trompouki, E.
- ID
- ZDB-PUB-181031-3
- Date
- 2018
- Source
- Frontiers in cell and developmental biology 6: 124 (Other)
- Registered Authors
- Trompouki, Eirini
- Keywords
- hematopoiesis, hematopoietic (stem) cells, next generation sequencing, technology, zebrafish
- MeSH Terms
- none
- PubMed
- 30374440 Full text @ Front Cell Dev Biol
Citation
de Pater, E., Trompouki, E. (2018) Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis. Frontiers in cell and developmental biology. 6:124.
Abstract
Hematopoiesis is an optimal system for studying stem cell maintenance and lineage differentiation under physiological and pathological conditions. In vertebrate organisms, billions of differentiated hematopoietic cells need to be continuously produced to replenish the blood cell pool. Disruptions in this process have immediate consequences for oxygen transport, responses against pathogens, maintenance of hemostasis and vascular integrity. Zebrafish is a widely used and well-established model for studying the hematopoietic system. Several new hematopoietic regulators were identified in genetic and chemical screens using the zebrafish model. Moreover, zebrafish enables in vivo imaging of hematopoietic stem cell generation and differentiation during embryogenesis, and adulthood. Finally, zebrafish has been used to model hematopoietic diseases. Recent technological advances in single-cell transcriptome analysis, epigenetic regulation, proteomics, metabolomics, and processing of large data sets promise to transform the current understanding of normal, abnormal, and malignant hematopoiesis. In this perspective, we discuss how the zebrafish model has proven beneficial for studying physiological and pathological hematopoiesis and how these novel technologies are transforming the field.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping