PUBLICATION
An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
- Authors
- Bresciani, E., Broadbridge, E., Liu, P.P.
- ID
- ZDB-PUB-181027-10
- Date
- 2018
- Source
- MethodsX 5: 1287-1290 (Other)
- Registered Authors
- Bresciani, Erica, Liu, Pu Paul
- Keywords
- Dissociation, Larvae dissociation to single cell suspension, Single cell suspension, Zebrafish, Zebrafish embryo
- MeSH Terms
- none
- PubMed
- 30364607 Full text @ MethodsX
Citation
Bresciani, E., Broadbridge, E., Liu, P.P. (2018) An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae. MethodsX. 5:1287-1290.
Abstract
Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping