ZFIN ID: ZDB-PUB-181026-8
Microinjection in Zebrafish for Genome Editing and Functional Studies
Pei, W., Burgess, S.M.
Date: 2019
Source: Methods in molecular biology (Clifton, N.J.)   1874: 459-474 (Other)
Registered Authors: Burgess, Shawn, Pei, Wuhong
Keywords: CRISPR, Functional studies, Microinjection, Mutagenesis, Transgenesis, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/growth & development
  • CRISPR-Cas Systems
  • Chaperonin 60/genetics
  • Gene Editing/methods*
  • Microinjections/methods*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 30353530
A major strength of zebrafish as a model organism is their rapid, in vitro development. The easy access to embryos compared to mammals, allows larval molecular and cellular composition to be manipulated by microinjection, providing a powerful avenue for biological and translational studies. Here, we describe the essential steps and different applications of microinjection in zebrafish for genome editing and functional studies, along with some experimental tips that are critical for microinjection success.