|ZFIN ID: ZDB-PUB-181026-8|
Microinjection in Zebrafish for Genome Editing and Functional Studies
Pei, W., Burgess, S.M.
|Source:||Methods in molecular biology (Clifton, N.J.) 1874: 459-474 (Other)|
|Registered Authors:||Burgess, Shawn, Pei, Wuhong|
|Keywords:||CRISPR, Functional studies, Microinjection, Mutagenesis, Transgenesis, Zebrafish|
Pei, W., Burgess, S.M. (2019) Microinjection in Zebrafish for Genome Editing and Functional Studies. Methods in molecular biology (Clifton, N.J.). 1874:459-474.
ABSTRACTA major strength of zebrafish as a model organism is their rapid, in vitro development. The easy access to embryos compared to mammals, allows larval molecular and cellular composition to be manipulated by microinjection, providing a powerful avenue for biological and translational studies. Here, we describe the essential steps and different applications of microinjection in zebrafish for genome editing and functional studies, along with some experimental tips that are critical for microinjection success.
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