PUBLICATION
Microinjection in Zebrafish for Genome Editing and Functional Studies
- Authors
- Pei, W., Burgess, S.M.
- ID
- ZDB-PUB-181026-8
- Date
- 2019
- Source
- Methods in molecular biology (Clifton, N.J.) 1874: 459-474 (Other)
- Registered Authors
- Burgess, Shawn, Pei, Wuhong
- Keywords
- CRISPR, Functional studies, Microinjection, Mutagenesis, Transgenesis, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/growth & development
- CRISPR-Cas Systems
- Chaperonin 60/genetics
- Gene Editing/methods*
- Microinjections/methods*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 30353530 Full text @ Meth. Mol. Biol.
Citation
Pei, W., Burgess, S.M. (2019) Microinjection in Zebrafish for Genome Editing and Functional Studies. Methods in molecular biology (Clifton, N.J.). 1874:459-474.
Abstract
A major strength of zebrafish as a model organism is their rapid, in vitro development. The easy access to embryos compared to mammals, allows larval molecular and cellular composition to be manipulated by microinjection, providing a powerful avenue for biological and translational studies. Here, we describe the essential steps and different applications of microinjection in zebrafish for genome editing and functional studies, along with some experimental tips that are critical for microinjection success.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping