PUBLICATION
Knockdown of chondroitin-4-sulfotransferase-1, but not of dermatan-4-sulfotransferase-1, accelerates regeneration of zebrafish after spinal cord injury
- Authors
- Sahu, S., Li, R., Loers, G., Schachner, M.
- ID
- ZDB-PUB-181020-30
- Date
- 2018
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33(2): 2252-2262 (Journal)
- Registered Authors
- Schachner, Melitta
- Keywords
- axonal regrowth, chondroitin sulfate, dermatan sulfate, glycosaminoglycans
- MeSH Terms
-
- Zebrafish
- Animals
- Gene Knockdown Techniques
- Chondroitin/metabolism*
- Sulfotransferases/genetics
- Sulfotransferases/metabolism*
- Spinal Cord Injuries/genetics*
- PubMed
- 30339470 Full text @ FASEB J.
Citation
Sahu, S., Li, R., Loers, G., Schachner, M. (2018) Knockdown of chondroitin-4-sulfotransferase-1, but not of dermatan-4-sulfotransferase-1, accelerates regeneration of zebrafish after spinal cord injury. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(2):2252-2262.
Abstract
Glycosaminoglycans such as chondroitin sulfate (CS) and dermatan sulfate (DS) are long chains of repeating disaccharide units, covalently linked to core proteins to form proteoglycans. Proteoglycans can be cell membrane-bound or are part of the extracellular matrix. They are important in a wide range of biologic processes, including development, synaptic plasticity, and regeneration after injury, as well as modulation of growth factor signaling, cell migration, survival, and proliferation. Synthesis of CS and DS in the Golgi apparatus is mediated by sulfotransferases that modify sugar chains through transfer of sulfate groups to specific positions on the sugar moieties. To clarify the functions of CS and DS during nervous system regeneration, we studied the effect of chondroitin 4- O-sulfotransferase-1/carbohydrate sulfotransferase-11 (C4ST-1/Chst-11) and dermatan 4- O-sulfotransferase-1/Chst-14 (D4ST-1/Chst-14) down-regulation on spinal cord regeneration in larval and adult zebrafish. In our study, knockdown of C4ST1/Chst-11 accelerated regeneration after spinal cord injury in larval and adult zebrafish and knockdown of D4ST1/Chst-14 did not alter regenerative capacity. From these and previous observations, we drew the conclusion that different CS and DS expression patterns can be growth permitting, growth inhibiting, or neutral for regrowing or sprouting axons, depending on the tissue environment of a particular animal species.-Sahu, S., Li, R., Loers, G., Schachner, M. Knockdown of chondroitin-4-sulfotransferase-1, but not of dermatan-4-sulfotransferase-1, accelerates regeneration of zebrafish after spinal cord injury.
Errata / Notes
This article is corrected by ZDB-PUB-220906-178 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping