PUBLICATION
Imaging of Endothelial Cell Dynamic Behavior in Zebrafish
- Authors
- Coxam, B., Gerhardt, H.
- ID
- ZDB-PUB-180923-9
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1846: 181-195 (Chapter)
- Registered Authors
- Coxam, Baptiste, Gerhardt, Holger
- Keywords
- Cell cycle, Endothelial cells, Kymograph, Membrane, Polarity, Spinning disk, Vasculature, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biomarkers
- Cell Cycle
- Cell Membrane/metabolism
- Cell Membrane/pathology
- Embryo, Nonmammalian
- Endothelial Cells/metabolism*
- Fluorescent Antibody Technique
- Genes, Reporter
- Intercellular Junctions
- Molecular Imaging*/methods
- Zebrafish*
- PubMed
- 30242760 Full text @ Meth. Mol. Biol.
Citation
Coxam, B., Gerhardt, H. (2018) Imaging of Endothelial Cell Dynamic Behavior in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1846:181-195.
Abstract
In recent years, use of the zebrafish embryo as a model organism to study vascular development in vivo has provided valuable insights into the genetic and cellular events shaping the embryonic vasculature. In this chapter, we aim to present the methods for the measurement of some of the most commonly investigated dynamic parameters in endothelial cells during developmental angiogenesis, namely, migration speed and acceleration, filopodia extension, front-rear polarity, cell cycle progression, membrane deformations, and junctional rearrangements. We also offer suggestions on how to deal with the most common imaging and quantifications challenges faced when acquiring and quantifying endothelial cell dynamic behavior in vivo.We intend this section to serve as an experience-based imaging primer for scientists interested in endothelial cell imaging in the zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping