PUBLICATION
Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy
- Authors
- Omar, M., Rebling, J., Wicker, K., Schmitt-Manderbach, T., Schwarz, M., Gateau, J., López-Schier, H., Mappes, T., Ntziachristos, V.
- ID
- ZDB-PUB-180902-3
- Date
- 2017
- Source
- Light, science & applications 6: e16186 (Journal)
- Registered Authors
- Lopez-Schier, Hernan
- Keywords
- deconvolution, development, mesoscopy, multiview, optoacoustics, photoacoustics
- MeSH Terms
- none
- PubMed
- 30167190 Full text @ Light Sci Appl
Citation
Omar, M., Rebling, J., Wicker, K., Schmitt-Manderbach, T., Schwarz, M., Gateau, J., López-Schier, H., Mappes, T., Ntziachristos, V. (2017) Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy. Light, science & applications. 6:e16186.
Abstract
Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping