Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis
- Kawahara, A., Morita, H., Yanagi, K., Ishizaka, T., Taimatsu, K., Ohga, R.
- Gene expression patterns : GEP 30: 32-36 (Journal)
- Registered Authors
- Kawahara, Atsuo, Morita, Hitoshi
- Floor plate, Hypochord, Liver, Pancreas, slurp-like1/ly2.3/ly97.3, slurp-like2/ly2.2/ly97.2
- MeSH Terms
- Amino Acid Sequence
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Gene Expression Regulation, Developmental*
- Sequence Homology
- Zebrafish Proteins/genetics*
- 30149151 Full text @ Gene Expr. Patterns
Kawahara, A., Morita, H., Yanagi, K., Ishizaka, T., Taimatsu, K., Ohga, R. (2018) Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis. Gene expression patterns : GEP. 30:32-36.
Mammalian SLURP1 and SLURP2 belong to the Ly-6/uPAR superfamily and are involved in maintaining the physiological integrity of keratinocytes. However, the developmental expression and functions of other Ly-6/uPAR family genes in vertebrates are still obscure. We have isolated novel Ly-6/uPAR family genes slurp-like1 (ly2.3/ly97.3) and slurp-like2 (ly2.2/ly97.2) in zebrafish. Both the Slurp-like1 and Slurp-like2 proteins contain the typical signal sequence and carboxy-terminal CCXXXXCN (X: an arbitrary amino acid) consensus sequence of the Ly-6/uPAR family but lack a transmembrane domain and a GPI-anchoring signal sequence, suggesting that both proteins may function as secretory proteins. Whole-mount in situ hybridization analysis revealed that slurp-like1 was predominantly expressed in the floor plate of the neural tube and in the hypochord of the notochord at 24 h post-fertilization (hpf) and detected in the liver and intestinal bulb at 72 hpf, while slurp-like2 was expressed in the midbrain and hindbrain at 24 hpf and detected in the liver and pancreas at 72 hpf. Differential expression profiles of the slurp-like1 and slurp-like2 genes suggest the distinct physiological involvement of these genes in zebrafish early embryogenesis.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes