PUBLICATION
Aeromonas veronii and aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinid fish
- Authors
- Ran, C., Qin, C., Xie, M., Zhang, J., Li, J., Xie, Y., Wang, Y., Li, S., Liu, L., Fu, X., Lin, Q., Li, N., Liles, M.R., Zhou, Z.
- ID
- ZDB-PUB-180824-7
- Date
- 2018
- Source
- Environmental microbiology 20(9): 3442-3456 (Journal)
- Registered Authors
- Li, Jie, Liu, Lihui, Qin, Chubin, Ran, Chao, Xie, Yadong, Zhang, Jinxiong, Zhou, Zhigang
- Keywords
- bacteria, microbe:higher organism interactions, pathogen ecology
- MeSH Terms
-
- Aeromonas/isolation & purification
- Aeromonas veronii/genetics
- Aeromonas veronii/metabolism*
- Aeromonas veronii/pathogenicity*
- Animals
- Bacterial Toxins/genetics
- Bacterial Toxins/metabolism*
- Bacterial Toxins/toxicity
- China
- Fish Diseases/microbiology*
- Gram-Negative Bacterial Infections/microbiology
- Gram-Negative Bacterial Infections/veterinary*
- Pore Forming Cytotoxic Proteins/genetics
- Pore Forming Cytotoxic Proteins/metabolism*
- Pore Forming Cytotoxic Proteins/toxicity
- Sepsis/microbiology
- Sepsis/veterinary*
- Virulence
- Virulence Factors/genetics
- Virulence Factors/metabolism
- Virulence Factors/toxicity
- Zebrafish/microbiology
- PubMed
- 30136361 Full text @ Environ. Microbiol.
Citation
Ran, C., Qin, C., Xie, M., Zhang, J., Li, J., Xie, Y., Wang, Y., Li, S., Liu, L., Fu, X., Lin, Q., Li, N., Liles, M.R., Zhou, Z. (2018) Aeromonas veronii and aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinid fish. Environmental microbiology. 20(9):3442-3456.
Abstract
Aeromonas species are ubiquitous inhabitants of freshwater environments, and are responsible for fish motile aeromonad septicemia (MAS). A. hydrophila is implicated as the primary etiologic agent of MAS. Here, we analyzed MAS epidemiological data for cyprinid fish in southern China, and found that A. veronii infections dominated. Consistent with this observation, A. veronii isolates were generally more virulent than A. hydrophila isolates when infecting germ-free zebrafish larvae via continuous immersion challenge. Through in vivo screening of the transposon library of the A. veronii strain Hm091, aerolysin was identified as the key virulence factor. Further results indicated that A. veronii Hm091 aerolysin disrupts the intestinal barrier of zebrafish, enabling systematic invasion by not only A. veronii Hm091 in a mono-infection, but also A. hydrophila NJ-1 in a mixed infection. Moreover, the differences in aerolysin expression and activity were the major contributor to the observed differences between the A. veronii and A. hydrophila strains regarding invasion efficacy via intestine. Together, our results provide new insights into the etiology and pathogenesis of Aeromonas infections, and highlight the importance of A. veronii-targeted treatments in future efforts against MAS. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping