PUBLICATION

Nodal signaling has dual roles in fate specification and directed migration during germ layer segregation

Authors
Liu, Z., Woo, S., Weiner, O.D.
ID
ZDB-PUB-180817-2
Date
2018
Source
Development (Cambridge, England)   145(17): (Journal)
Registered Authors
Woo, Stephanie
Keywords
Endoderm, Germ layer segregation, Ingression, Nodal signaling, Zebrafish gastrulation
MeSH Terms
  • Nodal Protein/genetics
  • Nodal Protein/metabolism*
  • SOX Transcription Factors/metabolism
  • Gastrulation/physiology*
  • Endoderm/embryology*
  • Endoderm/metabolism
  • Zebrafish Proteins/metabolism
  • Germ Layers/embryology*
  • Animals
  • Gene Expression Regulation, Developmental
  • Cell Movement/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Transforming Growth Factor beta/metabolism
(all 14)
PubMed
30111654 Full text @ Development
Abstract
During gastrulation, endodermal cells actively migrate to the interior of the embryo, but the signals that initiate and coordinate this migration are poorly understood. By transplanting ectopically induced endodermal cells far from the normal location of endoderm specification, we identified the inputs that drive internalization without the confounding influences of fate specification and global morphogenic movements. We find that Nodal signaling triggers an autocrine circuit for initiating endodermal internalization. Activation of the Nodal receptor directs endodermal specification through sox32 and also induces expression of more Nodal ligands. These ligands act in an autocrine fashion to initiate endodermal cell sorting. Our work defines an 'AND' gate consisting of sox32-dependent endodermal specification and Nodal ligand reception controlling endodermal cell sorting to the inner layer of the embryo at the onset of gastrulation.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s870TgTransgenic Insertion
    s903TgTransgenic Insertion
      sfc16TgTransgenic Insertion
        tud7TgTransgenic Insertion
          tz257
            		Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            aplnraMO7-aplnraMRPHLNO
            aplnrbMO1-aplnrbMRPHLNO
            ndr1MO4-ndr1MRPHLNO
            ndr2MO1-ndr2MRPHLNO
            prex1MO1-prex1MRPHLNO
            sox32MO1-sox32MRPHLNO
            1 - 6 of 6
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            Fish
            No data available
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            DsRed2EFGDsRed2
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
            1 - 5 of 5
            Show
            Mapping
            No data available
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            Citation
            Liu, Z., Woo, S., Weiner, O.D. (2018) Nodal signaling has dual roles in fate specification and directed migration during germ layer segregation. Development (Cambridge, England). 145(17):.