PUBLICATION
Social behavioral phenotyping of the zebrafish Casper mutant following embryonic alcohol exposure
- Authors
- Fernandes, Y., Rampersad, M., Eberhart, J.K.
- ID
- ZDB-PUB-180816-9
- Date
- 2018
- Source
- Behavioural brain research 356: 46-50 (Journal)
- Registered Authors
- Eberhart, Johann
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Brain/drug effects
- Disease Models, Animal
- Embryo, Nonmammalian/drug effects*
- Ethanol/adverse effects*
- Ethanol/pharmacology
- Female
- Fetal Alcohol Spectrum Disorders/metabolism
- Maternal-Fetal Exchange/drug effects
- Phenotype
- Pregnancy
- Social Behavior
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 30107225 Full text @ Behav. Brain Res.
Citation
Fernandes, Y., Rampersad, M., Eberhart, J.K. (2018) Social behavioral phenotyping of the zebrafish Casper mutant following embryonic alcohol exposure. Behavioural brain research. 356:46-50.
Abstract
The term Fetal Alcohol Spectrum Disorder (FASD) describes all the deleterious consequences of prenatal alcohol exposure. Impaired social behavior is a common symptom of FASD. The zebrafish has emerged as a powerful model organism with which to examine the effects of embryonic alcohol exposure on social behavior due to an innate strong behavior, called shoaling. The relative transparency of the embryo also makes zebrafish powerful for cellular analyses, such as characterizing neural circuitry. However, as zebrafish develop, pigmentation begins to obscure the brain and other tissues. Due to mutations disrupting pigmentation, the casper zebrafish strain remains relatively transparent throughout adulthood, potentially permitting researchers to image neural circuits in vivo, via epifluorescence, confocal and light sheet microscopy. Currently, however the behavioral profile of casper zebrafish post embryonic alcohol exposure has not been completed. We report that exposure to 1% alcohol from either 6 to 24, or 24 to 26 hours postfertilization reduces the social behavior of adult casper zebrafish. Our findings set the stage for the use of this important zebrafish resource in studies of FASD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping