|ZFIN ID: ZDB-PUB-180805-5|
Programmable base editing in zebrafish using a modified CRISPR-Cas9 system
Qin, W., Lu, X., Lin, S.
|Source:||Methods (San Diego, Calif.) 150: 19-23 (Journal)|
|Registered Authors:||Lin, Shuo|
|PubMed:||30076894 Full text @ Methods|
Qin, W., Lu, X., Lin, S. (2018) Programmable base editing in zebrafish using a modified CRISPR-Cas9 system. Methods (San Diego, Calif.). 150:19-23.
ABSTRACTThe use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human diseases that are caused by point mutations, a robust methodology for generating desirable DNA base changes is still needed. Recently, Cas9-linked cytidine deaminases (base editors) evolved as a strategy to introduce single base mutations in model organisms. They have been used to convert cytidine to thymine at specific genomic loci. Here we describe a protocol for using the base editing system in zebrafish and its application to reproduce a single base mutation observed in human Ablepharon-Macrostomia Syndrome.