PUBLICATION
Programmable base editing in zebrafish using a modified CRISPR-Cas9 system
- Authors
- Qin, W., Lu, X., Lin, S.
- ID
- ZDB-PUB-180805-5
- Date
- 2018
- Source
- Methods (San Diego, Calif.) 150: 19-23 (Journal)
- Registered Authors
- Lin, Shuo
- Keywords
- none
- MeSH Terms
-
- Abnormalities, Multiple/genetics
- Animals
- Base Sequence/genetics
- CRISPR-Cas Systems/genetics*
- Cytidine/metabolism
- Cytidine Deaminase/genetics
- Disease Models, Animal
- Embryo, Nonmammalian
- Eye Abnormalities/genetics
- Female
- Gene Editing/methods*
- Humans
- Macrostomia/genetics
- Male
- Mutagenesis, Site-Directed/methods
- Point Mutation/genetics
- RNA, Guide, Kinetoplastida
- Thymine/metabolism
- Twist-Related Protein 2/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 30076894 Full text @ Methods
Citation
Qin, W., Lu, X., Lin, S. (2018) Programmable base editing in zebrafish using a modified CRISPR-Cas9 system. Methods (San Diego, Calif.). 150:19-23.
Abstract
The use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human diseases that are caused by point mutations, a robust methodology for generating desirable DNA base changes is still needed. Recently, Cas9-linked cytidine deaminases (base editors) evolved as a strategy to introduce single base mutations in model organisms. They have been used to convert cytidine to thymine at specific genomic loci. Here we describe a protocol for using the base editing system in zebrafish and its application to reproduce a single base mutation observed in human Ablepharon-Macrostomia Syndrome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping