PUBLICATION
L-tyrosine supplementation does not ameliorate skeletal muscle dysfunction in zebrafish and mouse models of dominant skeletal muscle α-actin nemaline myopathy
- Authors
- Messineo, A.M., Gineste, C., Sztal, T.E., McNamara, E.L., Vilmen, C., Ogier, A.C., Hahne, D., Bendahan, D., Laing, N.G., Bryson-Richardson, R.J., Gondin, J., Nowak, K.J.
- ID
- ZDB-PUB-180802-4
- Date
- 2018
- Source
- Scientific Reports 8: 11490 (Journal)
- Registered Authors
- Bryson-Richardson, Robert, Sztal, Tamar Esther
- Keywords
- none
- MeSH Terms
-
- Myopathies, Nemaline/drug therapy*
- Myopathies, Nemaline/metabolism*
- Mice, Inbred C57BL
- Mutation/drug effects
- Muscle, Skeletal/drug effects*
- Muscle, Skeletal/metabolism*
- Male
- Dietary Supplements
- Disease Models, Animal
- Mice
- Actins/metabolism*
- Female
- Zebrafish/metabolism*
- Animals
- Tyrosine/administration & dosage*
- Energy Metabolism/drug effects
- PubMed
- 30065346 Full text @ Sci. Rep.
Citation
Messineo, A.M., Gineste, C., Sztal, T.E., McNamara, E.L., Vilmen, C., Ogier, A.C., Hahne, D., Bendahan, D., Laing, N.G., Bryson-Richardson, R.J., Gondin, J., Nowak, K.J. (2018) L-tyrosine supplementation does not ameliorate skeletal muscle dysfunction in zebrafish and mouse models of dominant skeletal muscle α-actin nemaline myopathy. Scientific Reports. 8:11490.
Abstract
L-tyrosine supplementation may provide benefit to nemaline myopathy (NM) patients, however previous studies are inconclusive, with no elevation of L-tyrosine levels in blood or tissue reported. We evaluated the ability of L-tyrosine treatments to improve skeletal muscle function in all three published animal models of NM caused by dominant skeletal muscle α-actin (ACTA1) mutations. Highest safe L-tyrosine concentrations were determined for dosing water and feed of wildtype zebrafish and mice respectively. NM TgACTA1D286G-eGFP zebrafish treated with 10 μM L-tyrosine from 24 hours to 6 days post fertilization displayed no improvement in swimming distance. NM TgACTA1D286G mice consuming 2% L-tyrosine supplemented feed from preconception had significant elevations in free L-tyrosine levels in sera (57%) and quadriceps muscle (45%) when examined at 6-7 weeks old. However indicators of skeletal muscle integrity (voluntary exercise, bodyweight, rotarod performance) were not improved. Additionally no benefit on the mechanical properties, energy metabolism, or atrophy of skeletal muscles of 6-7 month old TgACTA1D286G and KIActa1H40Y mice eventuated from consuming a 2% L-tyrosine supplemented diet for 4 weeks. Therefore this study yields important information on aspects of the clinical utility of L-tyrosine for ACTA1 NM.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping