ZFIN ID: ZDB-PUB-180730-3
Time-lapse imaging beyond the diffraction limit
Chitnis, A., Nogare, D.D.
Date: 2018
Source: Methods (San Diego, Calif.)   150: 32-41 (Other)
Registered Authors: Chitnis, Ajay
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/growth & development
  • Embryonic Development*
  • Intravital Microscopy/instrumentation
  • Intravital Microscopy/methods*
  • Microscopy, Fluorescence/instrumentation
  • Microscopy, Fluorescence/methods
  • Models, Animal
  • Time-Lapse Imaging/instrumentation
  • Time-Lapse Imaging/methods*
  • Zebrafish/growth & development*
PubMed: 30056120 Full text @ Methods
The zebrafish, with its rapid external development, optical transparency, and the relative ease with which transgenic lines can be created, is rapidly becoming the model of choice for examining developmental processes via time-lapse microscopy. The recent proliferation of techniques for super-resolution imaging now allows for an unprecedented view of embryonic development at high spatial and temporal resolution in live tissues. This review examines both the theoretical basis and practical application of a number of established and emerging super-resolution microscopy techniques, focusing on their application in time-lapse imaging of live zebrafish embryos.