PUBLICATION
Interactive effect of nitrogen source and high CO2 concentration on the growth of the dinoflagellate Alexandrium tamarense and its toxicity to zebrafish (Danio rerio) embryos.
- Authors
- Guan, W., Si, R., Li, X., Cai, J., Chen, S.
- ID
- ZDB-PUB-180726-1
- Date
- 2018
- Source
- Marine pollution bulletin 133: 626-635 (Journal)
- Registered Authors
- Li, Xi
- Keywords
- Alexandrium tamarense, CO(2) concentrations, Extracellular carbonic anhydrase (CA), Growth rate, Nitrogen (N) source, Toxicity
- MeSH Terms
-
- Animals
- Antioxidants
- Carbon Dioxide/metabolism*
- Carbon Dioxide/pharmacology
- Carbonic Anhydrases/metabolism
- Catalase/metabolism
- Dinoflagellida/drug effects
- Dinoflagellida/metabolism
- Dinoflagellida/physiology*
- Ecotoxicology/methods
- Embryo, Nonmammalian
- Glutathione Peroxidase/metabolism
- Glutathione Transferase/metabolism
- Nitrates
- Nitrogen/metabolism*
- Nitrogen/pharmacology
- Superoxide Dismutase/metabolism
- Zebrafish/embryology*
- PubMed
- 30041358 Full text @ Mar. Pollut. Bull.
Citation
Guan, W., Si, R., Li, X., Cai, J., Chen, S. (2018) Interactive effect of nitrogen source and high CO2 concentration on the growth of the dinoflagellate Alexandrium tamarense and its toxicity to zebrafish (Danio rerio) embryos.. Marine pollution bulletin. 133:626-635.
Abstract
The effects and interactive effects of different nitrogen (N) sources (ammonium, nitrate, and urea) and carbon dioxide (CO2) concentrations were investigated on Alexandrium tamarense, a harmful marine dinoflagellate, by measuring its growth (μ), extracellular carbonic anhydrase (CA), and its toxicity to zebrafish (Danio rerio) embryo. The μ and CA were influenced more strongly by CO2 concentrations rather than by N sources; significant effects of CO2 on μ and CA were observed under low CO2 concentration (LC) conditions compared to high CO2 concentration (HC) conditions. The ammonium and nitrate media under LC conditions had the maximum μ and CA, which was inhibited under HC conditions. The embryotoxic effects were influenced more strongly by the N sources than by CO2 concentrations, thus excluding the lower deformation in urea under HC conditions. Moreover, the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST), and catalase (CAT) were detected in normal (untreated) zebrafish embryos, and among them, the level of SOD was the highest. In summary, this study provides a clear insight for understanding the effects and interactive effects of N sources and CO2 concentrations on the growth and toxicity of harmful dinoflagellates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping